Russian Federation
Astana, Kazakhstan
Astana, Kazakhstan
Astana, Kazakhstan
Astana, Kazakhstan
Russian Federation
The object of the study was water samples, cultures of green microalgae Parachlorella kessleri U1 and Chlorella vulgaris I2, Maybalyk Lake (Astana). The aim and objectives of the study were to study the bioremediation potential of a consortium of two strains of microalgae Parachlorella kessleri U1 + Chlorella vulgaris I2 (hereinafter U1+I2) through the algolization of Lake Maibalyk according to microbiological and hydrochemical indicators. This lake is a large lake in Astana with fishery importance. The state of the reservoir is reflected in the state of the capital's air basin. A study of the water quality of Lake Maibalyk was conducted on the main morphometric, hydrochemical and hydrobiological indicators in May and June of 2020. To study the effect and determine the efficiency of using microalgae, preliminary biomass production and algolization of the U1 + I2 consortium were carried out. The introduction of a consortium of microalgae showed an improvement in the sanitary and microbiological indicators of the reservoir after just one month, due to its high antimicrobial properties and a decrease in such groups of bacteria as ammonifiers, coliforms, heterotrophic bacteria, coli group bacteria, pseudomonas, wax bacilli, staphylococcus, enterococci, enterobacteria, salmonella, fungi and yeast. After algolization for 30 days by the consortium I2+U1, an improvement was recorded not only in the organoleptic characteristics of water, but also in a number of hydrochemical parameters, including a decrease in COD, suspended solids, iron, SSAA and phosphates. . An increase in transparency, a decrease in water color and the absence of a specific odor were also noted. It was found that, although the content of BOD5, nitrates, sulfates in the original sample did not exceed the MРC values, the introduction of microalgae had a positive effect on reducing the concentration of these substances, which significantly affected the quality of the water in the reservoir. The results obtained can serve as a basis for developing environmentally friendly biotechnologies to improve water quality. In the future, it is necessary to conduct studies in triplicate, which will increase the reliability and quality of the data obtained, which will allow more effective assessment of the state of the ecosystem of the reservoir and ensure the safety of water use.
ozero Maybalyk, al'golizaciya, mikrovodorosli, zagryaznenie, kachestvo vody, biopreparat
1. Bogdanov, N.I. (2002). Chlorella – rezerv povysheniya produktivnosti zhivotnovodstva. Tsenovik, (4), 26. (In Russ.).
2. Bogdanov, N.I. (2008). Biologicheskaya reabilitatsiya vodoyemov. 3-ye izd., pererab. i dop. Penza: RIO PGSKHA., 126. (In Russ.). EDN: https://elibrary.ru/QKSSYX
3. Vayshlya, O.B., & Kulyatov, D.V. (2011). Perspektivnyye vidy mikrovodorosley dlya biodegradatsii pollyutantov vodnykh ekosistem yuga zapadnoy Sibiri // Izvestiya Samarskogo nauchnogo tsentra Rossiyskoy akademii nauk, 13, №1(4), 787-789. (In Russ.). EDN: https://elibrary.ru/OORXZL
4. Duktov, A.P., & Lavushev, V.I. (2022). Ekologiya akvakul'tury. Kurs lektsiy: uchebno-metodicheskoye posobiye. Gorki: BGSKHA, 103. (In Russ.).
5. Zakar'ya, K.D., Sarmurzina, Z.S., Bisenova, G.N., Urazova, M.S., Shakhabayeva, G.S., Abitayeva, G.K., & Abzhalelov, A.B. (2019). Biopreparat na osnove shtammov molochnokislykh probioticheskikh bakteriy dlya profilaktiki i lecheniya disbakteriozov, vyzvannymi bakterial'nymi vozbuditelyami, a takzhe ozdorovleniya i ochishcheniya i akvasredy. Patent RK 33967, 16 oktyabrya (In Russ.).
6. Zayadan, B.K., Sadvakasova, A.K., Kirbayeva, D.K., Bolatkhan, K., Salekh, M., & Bauyenova, M. (2013). Bezotkhodnaya tekhnologiya biologicheskoy ochistki stochnykh vod s pomoshch'yu mikrovodorosley. Vestnik KazNU. Seriya ekologicheskaya, №2/2 (38), 159-163. (In Russ.).
7. Kulnev, V.V., & Pochechun, V.A. (2016). Experience of algolization of drinking water bodies of the Nizhny Tagil industrial hub. Biosphere, 8, №3, 287-290. (In Russ.). EDN: https://elibrary.ru/WZISCJ
8. Melikhov, V.V., Kruzhilin, I.P., Kuznetsov, P.I., & Moskovets, M.V. (2008). Biologicheskaya melioratsiya presnovodnykh vodoyemov. Delovaya slava Rossii, 28-31. (In Russ.).
9. Mikhaylyuk, A.V. (2015). Pitatel'naya sreda Lyuka dlya kul'tivirovaniya mikrovodorosley. RU patent 2 556 126, 10 iyulya (In Russ.).
10. Proskurenko, I.V. (2003). Zamknutyye rybovodnyye ustanovki. Moskva: Izdatel'stvo VNI Rechn. i ozern. rybn. khoz-va,152. (In Russ.).
11. Sverchkova, N.V., Romanovskaya, T.V., & Samsonova, A.S. i dr. (2015). Konsortsium bakteriy – osnova preparata dlya obezzarazhivaniya i ochistki vody v prudakh i vodoyemakh. Sbornik nauchnykh trudov «Mikrobnyye biotekhnologii: Fundamental'nyye i Prikladnyye aspekty», (7), 445-457. (In Russ.).
12. Sainova, V.N., Katkov, I.S., & Sainov, D.I. (2018). Nekotoryye aspekty tekhnologii ochistki stochnykh vod ot biogennykh elementov. Bulatovskiye chteniya: Sbornik statey, 270-272. (In Russ.).
13. Safonova, Ye.F. (2004). Biodegradatsiya komponentov neftyanogo zagryazneniya s uchastiyem mikrovodorosley i tsianobakteriy: avtoref. kand. biol. nauk: Sankt-Peterburg. 21. (In Russ.). EDN: https://elibrary.ru/NIBIBD
14. Spott, S. (1983). Soderzhaniye ryb v zamknutykh sistemakh. Moskva. Legkaya i pishchevaya promyshlennost', 192. (In Russ.).
15. Tekebayeva Zh.B., Abzhalelov A.B., Abzhalelova L.A., & Ajtuganov K.A. (2017). Shtamm mikrovodorosli Parachlorella kessleri U1, ispol`zuemy`j dlya ochistki zagryaznenny`x prirodny`x vod ot razlichny`x pollyutantov. Patent KZ №103430 (In Russ.).
16. Tekebayeva Zh.B., Abzhalelov A.B., Abzhalelova L.A., & Ajtuganov K.A. (2017). Shtamm mikrovodorosli Chlorella vulgaris I2, ispol`zuemy`j dlya ochistki zagryaznenny`x prirodny`x vod ot razlichny`x pollyutantov. Patent KZ №103434 (In Russ.).
17. Trenkenshu, R.P., Borovkov, A.B., & Lelekov, A.S. (2009). Unifitsirovannaya laboratornaya ustanovka dlya issledovaniya nizshikh fototrofov, 41. (In Russ.). EDN: https://elibrary.ru/CQXHVR
18. Frolova, M.V., Komarova, O.P., & Moskovets, M.V. (2018). Sovremennaya biotekhnologiya v uluchshenii kachestva vody otkrytykh vodoyemov mnogotselevogo naznacheniya. Izvestiya Nizhnevolzhskogo agrouniversitetskogo kompleksa: Nauka i vyssheye professional'noye obrazovaniye, (4 (52)), 2013-218. (In Russ.). https://doi.org/10.32786/2071-9485-2018-04-30. EDN: https://elibrary.ru/YXTWCL
19. Shekhovtseva, N.V. (2008). Ekologiya vodnykh mikroorganizmov. Uchebnoye posobiye. Yarosl. Gos. un-t. Yaroslavl': Yar GU. 132. (In Russ.).
20. Alprol, A.E., Haneash, A.M.M., Ashour, M., Abualnaja, K.M., Alhashmialameer, D., Mansour, A.T., Sharawy, Z.Z., Abu-Saied, M.A., & Abomohra, A.E.-F. (2021). Potential application of Arthrospira platensis lipid-free biomass in bioremediation of organic dye from industrial textile effluents and its influence on marine rotifer (Brachionus plicatilis). Materials, 14 (16), 4446. https://doi.org/10.3390/ma14164446.
21. Burek, P., Satoh, Y., Fischer, G., Kahil, T., Nava Jimenez, L., Scherzer, A., Tramberend, S., Wada, Y., Eisner, S., Flörke, M., Hanasaki, N., Magnuszewski, P., Cosgrove, B., & Wiberg, D. (2016). Water Futures and Solution; Fast Track and Initiative. Final Report. ADA Project Number 2725-00/2014. IIASA: Laxenburg, Austria. 113. https://doi.org/10.5194/gmd-13-3267-2020. EDN: https://elibrary.ru/ZRGOXJ
22. Castillo-Carvajal, L.C., Sanz-Martin, J.L., & Barragan-Huerta, B.E. (2014). Biodegradation of organic pollutants in saline wastewater by halophilic microorganisms: a review. Environmental science and pollution research. 21(16), 9578-9588. https://doi.org/10.1007/s11356-014-3036-z. EDN: https://elibrary.ru/UOWOQD
23. Haripriya, U., Gopinath, K.P., Arun, J., & Govarthanan, M. (2022). Bioremediation of organic pollutants: A mini review on current and critical strategies for wastewater treatment. Arch. Microbiol., 204, 286. https://doi.org/10.1007/s00203-022-02907-9. EDN: https://elibrary.ru/ZZOSUK
24. Kube, M., Jefferson, B., Fan, L., & Roddick, F. (2018). The impact of wastewater characteristics, algal species selection and immobilisation on simultaneous nitrogen and phosphorus removal. Algal Res., 31, 478–488. https://doi.org/10.1016/j.algal.2018.01.009. EDN: https://elibrary.ru/VELORW
25. Pasqualino, J.C., Meneses, M., & Castells, F. (2011). Life Cycle Assessment of Urban Wastewater Reclamation and Reuse Alternatives. J. Ind. Ecol., 15, 49-63. https://doi.org/10.1111/j.1530-9290.2010.00293.x.
26. Rahman, M.S. (1992). Water quality management in aquaculture. Dhaka, BRAC Printers, 84.
27. Safi, C., Zebib, B., Merah, O., Pontalier, P.Y., & Vaca Garcia, C. (2014). Morphology, composition, production, processing and applications of Chlorella vulgaris: A review. Renewable and Sustainable Energy Reviews. 35, 265-278. https://doi.org/10.1016/j.rser.2014.04.007.
28. Singh, D.V., Kumar Upadhyay, A., Singh, R., & Singh, D.P. (2022). Persistent organic pollutants: Sources, impacts, and their remediation by microalgae. In Environmental Biotechnology. Apple Academic Press: New York, USA.
29. Schwarzenbach, R.P., Egli, T., Hofstetter, T.B., Von Gunten, U., & Wehrli, B. (2010). Global Water Pollution and Human Health. Annual Review of Environment and Resources, 35, 109-136. https://doi.org/10.1146/annurev-environ-100809-125342.
30. Usharani, K., Sruthilaya, K., & Divya, K. (2017). Determination of nitrate utilization efficiency of selective strain of Bacillus sp. Isolated from Eutrophic Lake, Theerthamkara, Kasaragod, Kerala. Pollution, 3 (1), 55-67. https://doi.org/10.7508/pj.2017.01.007.



